throughout the process of the experiment, there were several thing adjusted.
- The time for mice stand on the station will be defined as “found”. At the beginning of the experiment, the mice need to stay at the station for 5 second or longer to be recognize as “found”. However, we gradually noticed some of the mice won’t stay on the station for too long especially for the group injected coffin. The reason can be they are hyper active under the effect of coffin. The time changed from 5 second to 2 second after the first day.
- Instead of using pure coffin, I used the instant coffee substituted.
- After the experiment, the mice would reused by other experiment.
- During the lab, it was really critical to keep silence and non-light environment that to avoid all the factors could affect mice’s motion.